Dietary CML‐enriched protein induces functional arterial aging in a RAGE‐dependent manner in mice. Issue 5 (20th March 2015)

• Record Type: Journal Article
• Authors: Grossin, Nicolas; Auger, Florent; Niquet‐Leridon, Céline; Durieux, Nicolas; Montaigne, David; Schmidt, Ann Marie; Susen, Sophie; Jacolot, Philippe; Beuscart, Jean‐Baptiste; Tessier, Frédéric J.; Boulanger, Eric
• Is Part Of: Molecular nutrition & food research. Volume 59:Issue 5(2015:May)
• Page Start: 927
• Page End: 938
• Publication Date: 2015-03-20
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201400643
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2352-sec-0010" sec-type="section"> <title>Scope</title> <p>Advanced glycation end‐products (AGEs) are endogenously produced and are present in food. <italic>N</italic><sup>ε</sup>‐carboxymethyllysine (CML) is an endothelial activator via the receptor for AGEs (RAGEs) and is a major dietary AGE. This work investigated the effects of a CML‐enriched diet and RAGE involvement in aortic aging in mice.</p> </sec> <sec id="mnfr2352-sec-0020" sec-type="section"> <title>Methods and results</title> <p>After 9 months of a control diet or CML‐enriched diets (50, 100, or 200 μg<sub>CML</sub>/g of food), endothelium‐dependent relaxation, RAGE, vascular cell adhesion molecule‐1, and sirtuin‐1 expression, pulse wave velocity and elastin disruption were measured in aortas of wild‐type or RAGE<sup>−/−</sup> male C57BL/6 mice. Compared to the control diet, endothelium‐dependent relaxation was reduced in the wild‐type mice fed the CML‐enriched diet (200 μg<sub>CML</sub>/g) (66.8 ± 12.26 vs. 94.3 ± 2.6%, <italic>p</italic> < 0.01). RAGE and vascular cell adhesion molecule‐1 (<italic>p</italic> < 0.05) expression were increased in the aortic wall. RAGE<sup>−/−</sup> mice were protected against CML‐enriched diet‐induced endothelial dysfunction. Compared to control diet, the CML‐enriched diet (200 μg<sub>CML</sub>/g) increased the aortic pulse wave velocity (86.6 ± 41.1 vs. 251.4 ± 41.1 cm/s, <italic>p</italic> < 0.05) in wild‐type animals. Elastin disruption was found to a greater extent in the CML‐fed mice (<italic>p</italic> < 0.05). RAGE<sup>‐/−</sup> mice fed the CML‐enriched diet were protected from aortic stiffening.</p> </sec> <sec id="mnfr2352-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Chronic CML ingestion induced endothelial dysfunction, arterial stiffness and aging in a RAGE‐dependent manner.</p> </sec> </abstract>