Development of a feeding strategy for high cell and PHA density fed-batch fermentation of Ralstonia eutropha H16 from organic acids and their salts. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Huschner, Franz; Grousseau, Estelle; Brigham, Christopher J.; Plassmeier, Jens; Popovic, Milan; Rha, ChoKyun; Sinskey, Anthony J.
• Is Part Of: Process biochemistry. Volume 50:Issue 2(2015:Feb.)
• Page Start: 165
• Page End: 172
• Publication Date: 2015-02
• Subjects: Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2014.12.004
• Languages: English
• ISSNs: 1359-5113

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">A fed batch fermentation strategy was designed for <italic>Ralstonia eutropha</italic> using organic acids as feedstock.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">Organic acids were fed by pH-stat, and organic acid salts were fed using pO₂-dependent feed control.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">A fed batch strategy using three successive carbon/nitrogen ratios (10, 90, to infinite) was employed to maximize PHA productivity.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Biomass yield of 112.0 g/L, PHA yield of 93.5 g/L, and a PHA productivity of 2.13 g/L/h was achieved using the strategy outlined in this work.</p> </list-item> </list> </p> </sec> </abstract>