Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Zoglowek, Marta; Lübeck, Peter S.; Ahring, Birgitte K.; Lübeck, Mette
• Is Part Of: Process biochemistry. Volume 50:Issue 2(2015:Feb.)
• Page Start: 211
• Page End: 220
• Publication Date: 2015-02
• Subjects: Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2014.12.018
• Languages: English
• ISSNs: 1359-5113

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">Heterologous expression of CBHs in single hosts may produce multi-enzyme cocktails.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">Activity of heterologously expressed CBHs is (often) lower than of the native enzyme.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">Native inducible promoters are most successful for heterologous expression of CBHs.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Over-glycosylation of heterologously produced CBHs decreases enzyme activity.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Modulating protein quality control mechanisms in the host could improve CBH secretion.</p> </list-item> </list> </p> </sec> </abstract>