Dairy Debaryomyces hansenii strains produce the antihypertensive casein-derived peptides LHLPLP and HLPLP. Issue 2 (May 2015)

• Record Type: Journal Article
• Authors: García-Tejedor, Aurora; Sánchez-Rivera, Laura; Recio, Isidra; Salom, Juan B.; Manzanares, Paloma
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 61:Issue 2(2015:May)
• Page Start: 550
• Page End: 556
• Publication Date: 2015-05
• Subjects: Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2014.12.019
• Languages: English
• ISSNs: 0023-6438

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The ability of dairy <italic>Debaryomyces hansenii</italic>, <italic>Kluyveromyces lactis</italic> and <italic>Kluyveromyces marxianus</italic> strains to release the casein-derived antihypertensive sequences RYLGY, AYFYPEL, LHLPLP, HLPLP, VPP and/or IPP was examined. Yeast strains were grown in medium with casein as sole nitrogen source and the yeast casein hydrolysates (CSHs) were analysed by HPLC-MS/MS to search for the six antihypertensive sequences. Only LHLPLP and HLPLP were identified in CSHs and exclusively in <italic>D. hansenii</italic> Dh1 and Dh14 hydrolysates in which both antihypertensive sequences represented approximately 6 (CSH Dh1) and 10% (CSH Dh14) of total peptide content. In addition, a complete analysis of selected CSHs by HPLC-MS/MS allowed the identification of 35 (Dh1) and 46 (Dh14) additional peptides, which could also contribute to the observed <italic>in vitro</italic> ACE inhibitory potency of both hydrolysates (Dh1, IC<sub>50</sub> = 13.6 ± 1.8 μg/mL; Dh14, IC<sub>50</sub> = 17.5 ± 2.1 μg/mL) and might confer them multifunctional properties. Finally casein zymography revealed the presence of at least one extracellular protease with a molecular mass of about 50 kDa. In conclusion, the present study contributes to a better insight into bioactive compounds produced by dairy yeasts and shows the feasibility of <italic>D. hansenii</italic> strains to produce antihypertensive casein-derived peptides.</p> </sec> </abstract>