Production of isomalto-oligosaccharides by cell bound α-glucosidase of Microbacterium sp. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Ojha, Swati; Mishra, Saroj; Chand, Subhash
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 60:Issue 1(2015:Jan.)
• Page Start: 486
• Page End: 494
• Publication Date: 2015-01
• Subjects: Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2014.08.009
• Languages: English
• ISSNs: 0023-6438

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Isomalto-oligosaccharides (IMOs), one of the emerging prebiotics, were synthesized by transglycosylation activity of the cell bound α-glucosidase of a novel strain of <italic>Microbacterium</italic> sp. using maltose as a glycosyl donor. Parameters affecting yield were optimized and a yield of 85 g/l was obtained at 40% (w/v) initial substrate concentration after 24 h using whole cells (∼25 IU α-glucosidase activity). A mixture of isomaltotriose, isomaltotetraose, isomaltopentaose and isomaltohexaose were obtained as determined by electrospray ionization mass spectrometry. Addition of 0.5 M LiCl to the reaction mixture lead to a further 15% increase in the yield. The synthesized IMOs were purified for structure analysis by preparative thin layer chromatography and structures elucidated by ¹H and ¹³C NMR. The chemical shift values of ¹³C NMR clearly confirmed that the products formed were isomaltotriose and isomaltotetraose (α-1, 6 linked). The reactions were scaled up in a column to 100 ml level using immobilized cells. An increase in productivity by 4.2 times was achieved in this system with a conversion efficiency of 24%.</p> </sec> </abstract>