Intraspecific biodiversity and 'spoilage potential' of Brettanomyces bruxellensis in Apulian wines. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Di Toro, Maria Rosaria; Capozzi, Vittorio; Beneduce, Luciano; Alexandre, Hervé; Tristezza, Mariana; Durante, Miriana; Tufariello, Maria; Grieco, Francesco; Spano, Giuseppe
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 60:Issue 1(2015:Jan.)
• Page Start: 102
• Page End: 108
• Publication Date: 2015-01
• Subjects: Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2014.06.059
• Languages: English
• ISSNs: 0023-6438

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The yeast <italic>Brettanomyces bruxellensis</italic>, generally considered the main oenological spoilage microbe, is able to survive during the winemaking process and it confers off-odors to wine, in reason of its ability to produce considerable amounts of volatile phenols. Forty-eight isolates of <italic>B</italic>. <italic>bruxellensis</italic>, obtained from several wines collected in Apulia (Southern Italy), were genetically characterized using an integrated approach, including a strain biodiversity analysis by <italic>Sau</italic>-PCR. Furthermore, the production of volatile phenols was assessed in wine and in synthetic medium, confirming the oenological spoilage potential of the analysed strains. Our findings indicate a remarkable genetic variability of the <italic>B. bruxellensis</italic> identified strains and corroborate the evidence of a high level of genotypic and phenotypic polymorphism within <italic>B. bruxellensis</italic> species. Moreover, the observation reported suggest that strains from wines produced in the same geographical areas often clustered differently, indicating a complex intraspecific biodiversity in the regional wine environments. Diversity in volatile phenol production reflects intraspecific biodiversity highlighted by <italic>Sau</italic>-PCR. Strains diversity linked to differences in 'spoilage potential' increase the industrial relevance of this study, allowing the design of new strategies for <italic>B. bruxellensis</italic> control in wines.</p> </sec> </abstract>