Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Lee, Young-Duck; Park, Jong-Hyun
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 60:Issue 1(2015:Jan.)
• Page Start: 571
• Page End: 577
• Publication Date: 2015-01
• Subjects: Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2014.09.017
• Languages: English
• ISSNs: 0023-6438

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Two phages of <italic>Escherichia coli</italic> O157:H7 from a sewage sample were isolated and characterized to control <italic>E. coli</italic> O157:H7 in foods. The isolated phages, BECP2 and BECP6, belonged to the <italic>Myoviridae</italic> and <italic>Podoviridae, </italic> respectively. Their burst sizes were 33 PFU/cell for BECP2 and 51 PFU/cell for BECP6 after a latent period of 25 min. Shiga toxin genes were not detected on either the BECP2 or BECP6 phage. The two phages were susceptible to 70% ethanol. To determine the utility of the two phage mixture for biocontrol of <italic>E. coli</italic> O157:H7, we analyzed the reduction of <italic>E. coli</italic> O157:H7 in commercial milk and in biofilms. The mixture reduced the growth of <italic>E. coli</italic> O157:H7 from 6 log CFU/mL to 1 log CFU/mL after 5-h incubation at 37 °C in commercial milk. The mixture was applied to <italic>E. coli</italic> O157:H7 in a biofilm formed on glass wool, microtiter plate, and stainless steel coupon. The density of <italic>E. coli</italic> O157:H7 in the biofilm reduced from OD₆₀₀ 1.1 to 0.6 on the plate and from 6 log CFU/coupon to 2 log CFU/coupon on the coupon. Therefore, the BECP2 and BECP6 phages might be efficient sanitizing agents for reducing <italic>E. coli</italic> O157:H7 in a biofilm environment.</p> </sec> </abstract>