Identification of Sola l 4 as Bet v 1 homologous pathogenesis related‐10 allergen in tomato fruits. Issue 3 (8th February 2014)

• Record Type: Journal Article
• Authors: Wangorsch, Andrea; Jamin, Annette; Foetisch, Kay; Malczyk, Anna; Reuter, Andreas; Vierecke, Sabine; Schülke, Stefan; Bartel, Detlef; Mahler, Vera; Lidholm, Jonas; Vieths, Stefan; Scheurer, Stephan
• Is Part Of: Molecular nutrition & food research. Volume 59:Issue 3(2015:Mar.)
• Page Start: 582
• Page End: 592
• Publication Date: 2014-02-08
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201300620
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2167-sec-0010" sec-type="section"> <title>Scope</title> <p>The aim was to investigate the potential contribution of a major birch pollen Bet v 1‐homologous allergen to birch pollen‐associated tomato fruit allergy.</p> </sec> <sec id="mnfr2167-sec-0020" sec-type="section"> <title>Methods and results</title> <p>Two isoforms of a Bet v 1‐homologous protein (designated Sola l 4) from tomato fruit were identified by cDNA‐cloning and produced as recombinant proteins. Allergen‐specific IgE levels to tomato, birch pollen, Bet v 1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato‐ and Bet v 1‐specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Bet v 1 reacted with Sola l 4. Both Sola l 4 isoforms displayed allergenic potency and IgE‐cross‐reactivity with Bet v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse.</p> </sec> <sec id="mnfr2167-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Sola l 4, a novel pathogenesis related‐10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. IgE‐testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component‐resolved allergy diagnosis.</p> </sec> </abstract>