Dietary lipids modulate the expression of miR‐107, an miRNA that regulates the circadian system. Issue 3 (21st January 2015)

• Record Type: Journal Article
• Authors: Daimiel‐Ruiz, Lidia; Klett‐Mingo, Mercedes; Konstantinidou, Valentini; Micó, Victor; Aranda, Juan F.; García, Belén; Martínez‐Botas, Javier; Dávalos, Alberto; Fernández‐Hernando, Carlos; Ordovás, Jose M.
• Is Part Of: Molecular nutrition & food research. Volume 59:Issue 3(2015:Mar.)
• Page Start: 552
• Page End: 565
• Publication Date: 2015-01-21
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201400616
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2316-sec-0010" sec-type="section"> <title>Scope</title> <p>The increased prevalence of cardiovascular diseases (CVDs) has been hypothesized to be the result of an increased exposure to a host of atherogenic environmental factors, paramount among them being unhealthy dietary habits. Long‐chain n‐3 polyunsaturated fatty acids have been shown to have cardio protective effects, partially due to their ability to regulate gene expression. In this regard, increasing attention has been devoted to the role of miRNAs as regulators of multiple metabolic pathways whose deregulation has been associated with CVD risk.</p> </sec> <sec id="mnfr2316-sec-0020" sec-type="section"> <title>Methods and results</title> <p>In this work, we investigated whether miRNA expression was regulated by docosahexanoic acid, conjugated linoleic acid, and cholesterol in Caco‐2 cells. The modulated miRNAs, miR‐107 was differentially expressed by all treatments and this modulation was independent of its hosting gene, PANK1, possibly through its own promoter, which contains binding sites for metabolically relevant transcription factors. Among the putative target genes of miR‐107, we found some genes with key roles in circadian rhythm. Specifically, we demonstrated that binding of miR‐107 to the CLOCK gene results in the deregulation of the circadian rhythm of the cells.</p> </sec> <sec id="mnfr2316-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Since chronodisruption has been linked to metabolic disorders such as type 2 diabetes, atherosclerosis, obesity, and CVD, our findings suggests that miR‐107 could represent a new approach for pharmacological treatment of these diseases.</p> </sec> </abstract>