Augmentation of free amino acids in eggs of red snapper, Lutjanus campechanus as part of the induced spawning protocol. (29th March 2013)

• Record Type: Journal Article
• Authors: Hastey, Ryan; Phelps, Ronald; Davis, Allen; Cummins, Keith
• Is Part Of: Aquaculture research. Volume 46:Number 2(2015)
• Page Start: 283
• Page End: 290
• Publication Date: 2013-03-29
• Subjects: Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12184
• Languages: English
• ISSNs: 1355-557X

Abstract

<abstract abstract-type="main" id="are12184-abs-0001"> <title>Abstract</title> <p>An attempt was made to increase free amino acids (FAA) concentrations in eggs of red snapper <italic>Lutjanus campechanus</italic> as part of an induced spawning protocol. Mature female red snapper were given intramuscular injections of human chorionic gonadotropin (HCG) at a 1100 IU kg⁻¹ of female body weight. Immediately after the HCG injection, one set of females received an intramuscular injection of FAA in a buffered saline solution at a dose of 20 mg FAA cocktail kg⁻¹ of body weight. The FAA cocktail was 25% isoleucine, 25% leucine, 25% lysine and 25% valine on a molecular weight basis. A second set of fish did not receive the FAA supplement. Both sets of fish were equally successful in spawning with ovulation occurring 21–32.5 h post injection. Injection of FAA into brooders as eggs began final maturation and hydration resulted in an increase in leucine (nmoles mg egg⁻¹) and a slight increase in isoleucine (nmoles egg⁻¹) in recently ovulated eggs. FAA supplementation via brood injection altered FAA utilization rates by embryos and during larval development, resulted in a greater yolk sac and oil globule reserve in larvae as they approached the time of first feeding.</p> </abstract>