Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta. Issue 4 (28th October 2014)

• Record Type: Journal Article
• Authors: Chen, Qi‐Liang; Luo, Zhi; Huang, Chao; Zheng, Jia‐Lang; Pan, Ya‐Xiong; Song, Yu‐Feng; Hu, Wei
• Is Part Of: European journal of lipid science and technology. Volume 117:Issue 4(2015:Apr.)
• Page Start: 471
• Page End: 482
• Publication Date: 2014-10-28
• Subjects: Oils and fats, Edible -- Periodicals; Lipids -- Periodicals; 660.63
• DOI: 10.1002/ejlt.201400164
• Languages: English
• ISSNs: 1438-7697

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="ejlt201400164-sec-0001" sec-type="section"> <p>The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of <italic>Synechogobius hasta</italic>, including <italic>g6pd</italic>, <italic>6pgd</italic>, <italic>me</italic>, <italic>icdh</italic>, <italic>fas</italic>, <italic>accα</italic>, <italic>accβ</italic>, <italic>lpl</italic>, <italic>atgl</italic>, <italic>hsla</italic>, <italic>hslb</italic>, <italic>cpt 1a</italic>, <italic>srebp‐1</italic>, <italic>pparα</italic>, and <italic>pparγ</italic>. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of <italic>S. hasta</italic>. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels.</p> <p> <bold>Practical applications:</bold> Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles.</p> <p> <inline-graphic xlink:href="ark:/27927/pgjf84rj2d" content-type="ejlt201400164-gra-0001" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /> </p> <p>In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned and characterized from <italic>Synechogobius hasta</italic>, including <italic>g6pd</italic>, <italic>6pgd</italic>, <italic>me</italic>, <italic>icdh</italic>, <italic>fas</italic>, <italic>accα</italic>, <italic>accβ</italic>, <italic>lpl</italic>, <italic>atgl</italic>, <italic>hsla</italic>, <italic>hslb</italic>, <italic>cpt 1a</italic>, <italic>srebp‐1</italic>, <italic>pparα</italic>, and <italic>pparγ</italic>. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of <italic>S. hasta</italic>. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at the varying levels.</p> </sec> </abstract>