A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue. Issue 1 (27th February 2014)

• Record Type: Journal Article
• Authors: Sparks, Hugh; Warren, Sean; Guedes, Joana; Yoshida, Nagisa; Charn, Tze Choong; Guerra, Nadia; Tatla, Taranjit; Dunsby, Christopher; French, Paul
• Is Part Of: Journal of biophotonics. Volume 8:Issue 1/2(2015)
• Page Start: 168
• Page End: 178
• Publication Date: 2014-02-27
• Subjects: Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201300203
• Languages: English
• ISSNs: 1864-063X

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="jbio201300203-abs-0002"> <title> <x xml:space="preserve">Abstract</x> </title> <p xml:lang="de">Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. The study presents progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ~mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ~8 seconds using an average excitation power of ~0.5 mW at the specimen. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgh372pv5fm" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>