A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue. Issue 1 (27th February 2014) - Sparks, Hugh
Abstract Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. The study presents progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ~mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ~8 seconds using an average excitation power of ~0.5 mW at the specimen.