High dose of prebiotics reduces fecal water cytotoxicity in healthy subjects. Issue 11 (17th October 2014)

• Record Type: Journal Article
• Authors: Windey, Karen; François, Isabelle; Broekaert, Willem; De Preter, Vicky; Delcour, Jan A.; Louat, Thierry; Herman, Jean; Verbeke, Kristin
• Is Part Of: Molecular nutrition & food research. Volume 58:Issue 11(2014:Nov.)
• Page Start: 2206
• Page End: 2218
• Publication Date: 2014-10-17
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201400298
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2258-sec-0010" sec-type="section"> <title>Scope</title> <p>In vitro and animal studies have shown differential colonic fermentation of structurally different prebiotics. We evaluated the impact of two structurally different prebiotics (wheat bran extract (WBE, containing arabinoxylan‐oligosaccharides) and oligofructose) on colonic fermentation and markers of bowel health in healthy volunteers.</p> </sec> <sec id="mnfr2258-sec-0020" sec-type="section"> <title>Methods and results</title> <p>Nineteen healthy subjects completed a double‐blind, cross‐over randomized controlled trial. Interventions with WBE, oligofructose or placebo for 2 wk (week 1: 15 g/day; week 2: 30 g/day) were separated by 2‐wk wash‐out periods. At the end of each study period, colonic fermentation was characterized through a metabolomics approach. Fecal water genotoxicity and cytotoxicity were determined using the comet and WST‐1 assay, respectively, as parameters of gut health. Cluster analysis revealed differences in effects of WBE and oligofructose on colonic fermentation. WBE, but not oligofructose, reduced fecal p‐cresol (<italic>p</italic> = 0.009) and isovaleric acid concentrations (<italic>p</italic> = 0.022), markers of protein fermentation. Fecal water cytotoxicity was significantly lower after intake of WBE (<italic>p</italic> = 0.015). Both WBE‐ and oligofructose‐intake tended to reduce fecal water genotoxicity compared to placebo (WBE: <italic>p</italic> = 0.060; oligofructose: <italic>p</italic> = 0.057). Changes in fermentation were not related to changes in fecal water toxicity.</p> </sec> <sec id="mnfr2258-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Structurally different prebiotics affect colonic fermentation and gut health in a different way.</p> </sec> </abstract>