Changes in vitamin D target gene expression in adipose tissue monitor the vitamin D response of human individuals. Issue 10 (28th July 2014)

• Record Type: Journal Article
• Authors: Ryynänen, Jussi; Neme, Antonio; Tuomainen, Tomi‐Pekka; Virtanen, Jyrki K.; Voutilainen, Sari; Nurmi, Tarja; de Mello, Vanessa D. F.; Uusitupa, Matti; Carlberg, Carsten
• Is Part Of: Molecular nutrition & food research. Volume 58:Issue 10(2014:Oct.)
• Page Start: 2036
• Page End: 2045
• Publication Date: 2014-07-28
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201400291
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2232-sec-0010" sec-type="section"> <title>Scope</title> <p>Vitamin D<sub>3</sub>, its biologically most active metabolite 1α, 25‐dihydroxyvitamin D<sub>3</sub> (1, 25(OH)<sub>2</sub>D<sub>3</sub>), and the vitamin D receptor (VDR) are important for adipose tissue biology.</p> </sec> <sec id="mnfr2232-sec-0020" sec-type="section"> <title>Methods and results</title> <p>We extrapolated genomic VDR association loci in adipocytes from 55 conserved genome‐wide VDR‐binding sites in nonfat tissues. Taking the genes <italic>DUSP10</italic>, <italic>TRAK1</italic>, <italic>NRIP1</italic>, and <italic>THBD</italic> as examples, we confirmed the predicted VDR binding sites upstream of their transcription start sites and showed rapid mRNA up‐regulation of all four genes in SGBS human pre‐adipocytes. Using adipose tissue biopsy samples from 47 participants of a 5‐month vitamin D<sub>3</sub> intervention study, we demonstrated that all four primary VDR target genes can serve as biomarkers for the vitamin D<sub>3</sub> responsiveness of human individuals. Changes in <italic>DUSP10</italic> gene expression appear to be the most comprehensive marker, while <italic>THBD</italic> mRNA changes characterized a rather different group of study participants.</p> </sec> <sec id="mnfr2232-sec-0030" sec-type="section"> <title>Conclusion</title> <p>We present a new approach to predict vitamin D target genes based on conserved genomic VDR‐binding sites. Using human adipocytes as examples, we show that such ubiquitous VDR target genes can be used as markers for the individual's response to a supplementation with vitamin D<sub>3</sub>.</p> </sec> </abstract>