An Analysis of Escherichia coli Isolations for Antimicrobial Resistance Genes. Issue 3 (20th May 2014)

• Record Type: Journal Article
• Authors: Colobătiu, Liora; Oniga, Ovidiu; Tăbăran, Alexandra; Mihaiu, Romolica; Mirel, Simona; Daniel, Sorin Dan; Mihaiu, Marian
• Is Part Of: Journal of food safety. Volume 34:Issue 3(2014:Jul.)
• Page Start: 233
• Page End: 238
• Publication Date: 2014-05-20
• Subjects: Food adulteration and inspection -- Periodicals; Food contamination -- Periodicals; Food -- Analysis -- Periodicals; Food -- Microbiology -- Periodicals; Pathogenic bacteria -- Periodicals; Food handling -- Periodicals; Food preservatives -- Periodicals; 664
• DOI: 10.1111/jfs.12118
• Languages: English
• ISSNs: 0149-6085

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jfs12118-sec-0001" sec-type="section"> <p>This research was conducted in order to reveal antimicrobial resistance patterns and the prevalence of genes encoding resistance in <italic>E</italic><italic>scherichia coli</italic> isolated from raw poultry meat.</p> </sec> <sec id="jfs12118-sec-0002" sec-type="section"> <p>One hundred samples of poultry meat were collected, and 51 isolates of <italic>E</italic><italic>. coli</italic> were recovered and further tested for antimicrobial susceptibility to 11 antimicrobials, using the classical disk diffusion method. Fifteen isolates, showing a high degree of resistance among the collection were examined for antimicrobial resistance genes by multiplex polymerase chain reaction.</p> </sec> <sec id="jfs12118-sec-0003" sec-type="section"> <p>Resistance was most frequently observed to tetracycline (84.26%), ampicillin (73.03%) and sulfamethoxazole (66.29%). The tetracycline resistance genes <italic>TetA</italic> and <italic>TetB</italic> and beta‐lactam TEM gene were frequently detected in the tested isolates. Genes responsible for resistance to sulfonamides (33.33%) and trimethoprim (26.66%) were also found.</p> </sec> <sec id="jfs12118-sec-0004" sec-type="section"> <p> <italic>E</italic> <italic>. coli</italic> isolates recovered from raw poultry meat are highly resistant to antimicrobials and carry antimicrobial resistance genes that could be transferred to other microbes in the food chain, even important human pathogens.</p> </sec> <sec id="jfs12118-sec-0005" sec-type="section"> <title>Practical Applications</title> <p>The findings from this work provide baseline data for further antimicrobial resistance studies especially in our country, were antimicrobial resistance epidemiology needs constant improvement.</p> </sec> <sec id="jfs12118-sec-0006" sec-type="section"> <p>To our knowledge, this is one of the first reports regarding the identification of various resistance genes identified in <italic>E</italic><italic>. coli</italic> meat isolations in Romania. Moreover, the work contributes to the risk assessment concerning the possible <italic>E</italic><italic>. coli</italic> threat in food products and draws a warning signal for further actions that can assist the implementation of efficient measures to prevent possible adverse public health consequences.</p> </sec> </abstract>