Dynamics of Intact Immunoglobulin G Explored by Drift‐Tube Ion‐Mobility Mass Spectrometry and Molecular Modeling1. Issue 30 (10th June 2014)

• Record Type: Journal Article
• Authors: Pacholarz, Kamila J.; Porrini, Massimiliano; Garlish, Rachel A.; Burnley, Rebecca J.; Taylor, Richard J.; Henry, Alistair J.; Barran, Perdita E.
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 30(2014)
• Page Start: 7765
• Page End: 7769
• Publication Date: 2014-06-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201402863
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Collision cross‐sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift‐tube ion‐mobility mass spectrometry. Intact antibodies and Fc‐hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.</p> </abstract>