Dynamics of Intact Immunoglobulin G Explored by Drift‐Tube Ion‐Mobility Mass Spectrometry and Molecular Modeling1. Issue 30 (10th June 2014) - Pacholarz, Kamila J.
Abstract Collision cross‐sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift‐tube ion‐mobility mass spectrometry. Intact antibodies and Fc‐hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.