Ochratoxin A biocontrol and biodegradation by Bacillus subtilis CW 14. (31st December 2013)

• Record Type: Journal Article
• Authors: Shi, Lei; Liang, Zhihong; Li, Junxia; Hao, Junran; Xu, Yuancong; Huang, Kunlun; Tian, Jingjing; He, Xiaoyun; Xu, Wentao
• Is Part Of: Journal of the science of food and agriculture. Volume 94:Number 9(2014:Jul. 15)
• Page Start: 1879
• Page End: 1885
• Publication Date: 2013-12-31
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6507
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main" id="jsfa6507-abs-0001"> <title>Abstract</title> <sec id="jsfa6507-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jsfa6507-para-0001"> <bold>Ochratoxin A (OTA) is a mycotoxin produced by some <italic>Aspergillus</italic> and <italic>Penicillium</italic> species. In this study a strain of <italic>Bacillus subtilis</italic> was tested for its effects on OTA‐producing <italic>Aspergillus</italic> and OTA degradation. The mechanisms of the effects were also investigated</bold>.</p> </sec> <sec id="jsfa6507-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jsfa6507-para-0002"> <bold>A strain of <italic>Bacillus</italic> spp. isolated from fresh elk droppings was screened out using the methods described by Guan <italic>et al</italic>. (<italic>Int J Mol Sci</italic> 9:1489–1503 (2008)). The 16S rRNA gene sequence suggested that it was <italic>B. subtilis</italic> CW 14. It could inhibit the growth of the OTA‐producing species <italic>Aspergillus ochraceus</italic> 3.4412 and <italic>Aspergillus carbonarius</italic>, with inhibition rates of 33.0 and 33.3% respectively. At 6 µg mL<sup>−1</sup> OTA, both viable and autoclaved (121 °C, 20 min) cells of CW 14 bound more than 60% of OTA. In addition, OTA was degraded by the cell‐free supernatant of CW 14. By high‐performance liquid chromatography, the cell‐free supernatant degraded 97.6% of OTA after 24 h of incubation at 30 °C, and no degradation products were produced. The fastest degradation occurred during the first 2 h. In 3 g samples of contaminated maize, 47.1% of OTA was degraded by 50 mL inocula of overnight cultures of CW 14</bold>.</p> </sec> <sec id="jsfa6507-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jsfa6507-para-0003"> <bold>These findings indicated that <italic>B. subtilis</italic> CW 14 could both prevent OTA contamination and degrade OTA in crops. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>