A nano‐LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential. Issue 11 (20th March 2014)

• Record Type: Journal Article
• Authors: Rocco, Anna; Fanali, Chiara; Dugo, Laura; Mondello, Luigi; Cifuentes, Alejandro
• Is Part Of: Electrophoresis. Volume 35:Issue 11(2014:Jun.)
• Page Start: 1701
• Page End: 1708
• Publication Date: 2014-03-20
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201300621
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In this study, a simple and rapid methodology to analyze and quantify principal flavanones in citrus fruit juices through the use of a nano‐LC/UV‐Vis apparatus, employing a 75 μm id capillary column packed with sub‐2 μm particles C18 stationary phase for 10 cm, was developed. All compounds were baseline resolved working with a step gradient elution mode in 10 min. The developed analytical method was validated and the resulting RSD% for intra‐ and interday repeatability, related to retention time and peak area, were &lt;4.7 and 5.5%, respectively. LOD and LOQ values corresponded to 0.40 and 1.56 μg/mL for didymin, hesperitin, and narinegenin, while for the other flavanones were 0.78 and 3 μg/mL, respectively. Good linearity with acceptable determination coefficients <italic>R</italic>² was obtained in the range between LOQ concentration and 200 μg/mL (500 μg/mL naringin and hesperidin). Good recovery values were also obtained. Then, the method was applied to the analysis of selected hand‐squeezed and commercial citrus juices. Further, the nano‐LC system was coupled to a mass spectrometer to confirm analyte identification. Antioxidant capacity of selected samples was also evaluated measured by Folin–Ciocalteu assay and Trolox equivalent antioxidant capacity assay. Results were compared to determine total flavanones concentrations.</p> </abstract>