An aqueous pomegranate seed extract ameliorates oxidative stress of human hepatoma HepG2 cells. (3rd December 2013)

• Record Type: Journal Article
• Authors: Navarro, Marta; Amigo‐Benavent, Miryam; Mesias, Marta; Baeza, Gema; Gökmen, Vural; Bravo, Laura; Morales, Francisco J
• Is Part Of: Journal of the science of food and agriculture. Volume 94:Number 8(2014:Jun. 15)
• Page Start: 1622
• Page End: 1627
• Publication Date: 2013-12-03
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6469
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main" id="jsfa6469-abs-0001"> <title>Abstract</title> <sec id="jsfa6469-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jsfa6469-para-0001"> <bold>Aqueous pomegranate seed extract (PSE), a by‐product of the pomegranate juice industry, was recently identified as a potential antiglycative ingredient. Ellagic acid was proposed as the major polyphenol responsible for the antiglycative activity as exerted in <italic>in vitro</italic> models. However, there is no information on safety aspects of this extract in biological systems before its application as ingredient. The cytotoxicity of PSE (1–100 µg mL<sup>−1</sup>) was evaluated by determining its effect on cell viability and redox status of cultured HepG2 cells. The protective effect of the PSE against oxidative stress induced by <italic>tert</italic>‐butyl hydroperoxide (<italic>t</italic>‐BOOH) was also investigated</bold>.</p> </sec> <sec id="jsfa6469-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jsfa6469-para-0002"> <bold>No changes in cell integrity or intrinsic antioxidant status resulted from a direct treatment with aqueous PSE, even at high dosage. In addition, reactive oxygen species (ROS) induced by <italic>t</italic>‐BOOH were reduced by 21% when cells were pretreated with 100 µg mL<sup>−1</sup> of aqueous PSE at 180 min. The range of concentrations investigated was effective in decreasing the ROS formation but not in a dose‐dependent manner</bold>.</p> </sec> <sec id="jsfa6469-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jsfa6469-para-0003"> <bold>Aqueous pomegranate seed extract enhances human hepatoma cells integrity and resistance to cope with a stressful situation at concentration up to 100 µg mL<sup>−1</sup>. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>