Application of response surface methodology to optimise supercritical carbon dioxide extraction of volatile compounds from Crocus sativus. (28th October 2013)

• Record Type: Journal Article
• Authors: Shao, Qingsong; Huang, Yuqiu; Zhou, Aicun; Guo, Haipeng; Zhang, Ailian; Wang, Yong
• Is Part Of: Journal of the science of food and agriculture. Volume 94:Number 7(2014:May 15)
• Page Start: 1430
• Page End: 1436
• Publication Date: 2013-10-28
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6435
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main" id="jsfa6435-abs-0001"> <title>Abstract</title> <sec id="jsfa6435-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jsfa6435-para-0001"> <bold> <italic>Crocus sativus</italic> has been used as a traditional Chinese medicine for a long time. The volatile compounds of <italic>C. sativus</italic> appear biologically active and may act as antioxidants as well as anticonvulsants, antidepressants and antitumour agents. In order to obtain the highest possible yield of essential oils from <italic>C. sativus</italic>, response surface methodology was employed to optimise the conditions of supercritical fluid carbon dioxide extraction of the volatile compounds from <italic>C. sativus</italic>. Four factors were investigated: temperature, pressure, extraction time and carbon dioxide flow rate. Furthermore, the chemical compositions of the volatile compounds extracted by supercritical fluid extraction were compared with those obtained by hydro‐distillation and Soxhlet extraction</bold>.</p> </sec> <sec id="jsfa6435-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jsfa6435-para-0002"> <bold>The optimum extraction conditions were found to be: optimised temperature 44.9°C, pressure 34.9 MPa, extraction time 150.2 min and CO<sub>2</sub> flow rate 10.1 L h<sup>−1</sup>. Under these conditions, the mean extraction yield was 10.94 g kg<sup>−1</sup>. The volatile compounds extracted by supercritical fluid extraction and Soxhlet extraction contained a large amount of unsaturated fatty acids</bold>.</p> </sec> <sec id="jsfa6435-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jsfa6435-para-0003"> <bold>Response surface methodology was successfully applied for supercritical fluid CO<sub>2</sub> extraction optimisation of the volatile compounds from <italic>C. sativus</italic>. The study showed that pressure and CO<sub>2</sub> flow rate had significant effect on volatile compounds yield produced by supercritical fluid extraction. This study is beneficial for the further research operating on a large scale. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>