Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells1. Issue 52 (8th November 2013)

• Record Type: Journal Article
• Authors: Xiao, Han; Chatterjee, Abhishek; Choi, Sei‐hyun; Bajjuri, Krishna M.; Sinha, Subhash C.; Schultz, Peter G.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 52(2013)
• Page Start: 14080
• Page End: 14083
• Publication Date: 2013-11-08
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201308137
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>An enhanced suppression system</bold> enables the expression of proteins in mammalian cells incorporating one unnatural amino acid (UAA) into multiple sites, as well as two different UAAs into distinct sites in a protein of interest. The utility of this technology was demonstrated by generating a full‐length antibody, site‐specifically conjugated to a drug and a fluorophore, and characterizing its activity in vitro (see picture).<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4t3hxzzd" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>