Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells1. Issue 52 (8th November 2013) - Xiao, Han
Abstract An enhanced suppression system enables the expression of proteins in mammalian cells incorporating one unnatural amino acid (UAA) into multiple sites, as well as two different UAAs into distinct sites in a protein of interest. The utility of this technology was demonstrated by generating a full‐length antibody, site‐specifically conjugated to a drug and a fluorophore, and characterizing its activity in vitro (see picture).