Efficient identification of Malassezia yeasts by matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS). (February 2014)

• Record Type: Journal Article
• Authors: Kolecka, A.; Khayhan, K.; Arabatzis, M.; Velegraki, A.; Kostrzewa, M.; Andersson, A.; Scheynius, A.; Cafarchia, C.; Iatta, R.; Montagna, M.T.; Youngchim, S.; Cabañes, F.J.; Hoopman, P.; Kraak, B.; Groenewald, M.; Boekhout, T.
• Is Part Of: British journal of dermatology. Volume 170:Number 2(2014:Feb.)
• Page Start: 332
• Page End: 341
• Publication Date: 2014-02
• Subjects: Dermatology -- Periodicals; Skin -- Diseases -- Periodicals; 616.5
• DOI: 10.1111/bjd.12680
• Languages: English
• ISSNs: 0007-0963

Abstract

<abstract abstract-type="short" xml:lang="en" id="bjd12680-abs-0002"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>What's already known about this topic?</bold> </p> <p> <list id="bjd12680-list-0001" list-type="bullet"> <list-item> <p>Matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS) has been developed for the rapid identification of microorganisms and proved to be a robust tool for the recognition of bacterial species.</p> </list-item> <list-item> <p>Recently, it was shown that MALDI‐TOF MS provided reliable identification of yeasts and filamentous fungi.</p> </list-item> <list-item> <p>Identification of <italic>Malassezia</italic> isolates by routine methods remains laborious and results in a relatively high number of erroneous results.</p> </list-item> </list> </p> <p> <bold>What does this study add?</bold> </p> <p> <list id="bjd12680-list-0002" list-type="bullet"> <list-item> <p>A database of reference mass spectra resulting in efficient and correct identification of all <italic>Malassezia</italic> species by MALDI‐TOF MS in a short turnaround time.</p> </list-item> <list-item> <p>MALDI‐TOF MS results were consistent with those obtained by sequence analyses.</p> </list-item> <list-item> <p>The method may efficiently demonstrate the involvement of different <italic>Malassezia</italic> species in skin diseases.</p> </list-item> <list-item> <p>Application of MALDI‐TOF MS for the identification of <italic>Malassezia</italic> isolates is of clinical importance as fast and reliable identification will result in early treatment.</p> </list-item> </list> </p> </abstract>