Efficient identification of Malassezia yeasts by matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS). (February 2014) - Kolecka, A.
Abstract What's already known about this topic? Matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS) has been developed for the rapid identification of microorganisms and proved to be a robust tool for the recognition of bacterial species. Recently, it was shown that MALDI‐TOF MS provided reliable identification of yeasts and filamentous fungi. Identification of Malassezia isolates by routine methods remains laborious and results in a relatively high number of erroneous results. What does this study add? A database of reference mass spectra resulting in efficient and correct identification of all Malassezia species by MALDI‐TOF MS in a short turnaround time. MALDI‐TOF MS results were consistent with those obtained by sequence analyses. The method may efficiently demonstrate the involvement of different Malassezia species in skin diseases. Application of MALDI‐TOF MS for the identification of Malassezia isolates is of clinical importance as fast and reliable identification will result in early treatment.