Vig r 6, the cytokinin‐specific binding protein from mung bean (Vigna radiata) sprouts, cross‐reacts with Bet v 1‐related allergens and binds IgE from birch pollen allergic patients' sera. Issue 3 (1st September 2013)

• Record Type: Journal Article
• Authors: Guhsl, Eva Elisabeth; Hofstetter, Gerlinde; Hemmer, Wolfgang; Ebner, Christof; Vieths, Stefan; Vogel, Lothar; Breiteneder, Heimo; Radauer, Christian
• Is Part Of: Molecular nutrition & food research. Volume 58:Issue 3(2014:Mar.)
• Page Start: 625
• Page End: 634
• Publication Date: 2013-09-01
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201300153
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2070-sec-0010" sec-type="section"> <title>Scope</title> <p>Birch pollen associated allergy to mung bean sprouts is caused by cross‐reactivity between the birch pollen allergen Bet v 1 and the mung bean allergen Vig r 1. We aimed to determine the allergenicity of the cytokinin‐specific binding protein from mung bean (Vig r 6), another allergen related to Bet v 1 with only 31% sequence identity.</p> </sec> <sec id="mnfr2070-sec-0020" sec-type="section"> <title>Methods and results</title> <p>Bet v 1, Gly m 4, Vig r 1, and Vig r 6 were produced in <italic>Escherichia coli</italic>. In an ELISA, 73 and 32% of Bet v 1‐sensitized birch‐allergic patients' sera (<italic>n</italic> = 60) showed IgE binding to Vig r 1 and Vig r 6, respectively. Of 19 patients who reported allergic reactions or had positive prick‐to‐prick tests to mung bean sprouts, 79% showed IgE binding to Vig r 1 and 63% showed IgE binding to Vig r 6. Bet v 1 completely inhibited IgE binding to both mung bean allergens. Vig r 6 showed partial cross‐reactivity with Vig r 1 and activated basophils sensitized with mung bean allergic patients' sera.</p> </sec> <sec id="mnfr2070-sec-0030" sec-type="section"> <title>Conclusion</title> <p>We demonstrated IgE cross‐reactivity despite low sequence identity between Vig r 6 and other Bet v 1‐related allergens. Thus, IgE binding to Vig r 6 may contribute to birch pollinosis‐associated mung bean sprout allergy.</p> </sec> </abstract>