Maternal vitamin D deficiency causes smaller muscle fibers and altered transcript levels of genes involved in protein degradation, myogenesis, and cytoskeleton organization in the newborn rat. Issue 2 (21st August 2013)

• Record Type: Journal Article
• Authors: Max, Daniela; Brandsch, Corinna; Schumann, Sarah; Kühne, Hagen; Frommhagen, Matthias; Schutkowski, Alexandra; Hirche, Frank; Staege, Martin S.; Stangl, Gabriele I.
• Is Part Of: Molecular nutrition & food research. Volume 58:Issue 2(2014:Feb.)
• Page Start: 343
• Page End: 352
• Publication Date: 2013-08-21
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201300360
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2059-sec-0010" sec-type="section"> <title>Scope</title> <p>Epidemiologic data reveal associations between low serum concentrations of 25‐hydroxyvitamin D (25(OH)D) and higher risk of falls and muscle weakness. Fetal stage is critical for the development of skeletal muscle, but little information is available on the impact of maternal vitamin D deficiency on muscles of offspring.</p> </sec> <sec id="mnfr2059-sec-0020" sec-type="section"> <title>Methods and results</title> <p>To investigate the morphology and transcriptome of gastrocnemius muscle in newborns in response to maternal vitamin D deficiency, 14 female rats were fed either a vitamin D<sub>3</sub> deficient (0 IU/kg) or a vitamin D<sub>3</sub> adequate diet (1000 IU/kg) 8 weeks prior to conception, during pregnancy, and lactation. Analysis of cholecalciferol, 25(OH)D<sub>3</sub> and 1, 25‐dihydroxyvitamin D<sub>3</sub> show that dams fed the vitamin D deficient diet and their newborns suffered from a relevant vitamin D deficiency. Muscle cells of vitamin D deficient newborns were smaller than those of vitamin D adequate newborns (<italic>p</italic> < 0.05). Muscle transcriptome of the newborns revealed 426 probe sets as differentially expressed (259 upregulated, 167 downregulated) in response to vitamin D deficiency (fold change ≥1.5, <italic>p</italic> < 0.05). The effected genes are involved in protein catabolism, cell differentiation and proliferation, muscle cell development, and cytoskeleton organization.</p> </sec> <sec id="mnfr2059-sec-0030" sec-type="section"> <title>Conclusion</title> <p>Maternal vitamin D deficiency has a major impact on morphology and gene expression profile of skeletal muscle in newborns.</p> </sec> </abstract>