Isolation, Identification and Virulence Gene Profiling of Escherichia coli O157:H7 in Retail Doner Kebabs, Iran. Issue 4 (November 2013)

• Record Type: Journal Article
• Authors: Hosseini, Hedayat; Jamshidi, Abdollah; Basssami, Mohammad Reza; Khaksar, Ramin; Zeynali, Tayebe; Mousavi Khaneghah, Amin; Khanzadi, Saeid
• Is Part Of: Journal of food safety. Volume 33:Issue 4(2013:Oct.)
• Page Start: 489
• Page End: 496
• Publication Date: 2013-11
• Subjects: Food adulteration and inspection -- Periodicals; Food contamination -- Periodicals; Food -- Analysis -- Periodicals; Food -- Microbiology -- Periodicals; Pathogenic bacteria -- Periodicals; Food handling -- Periodicals; Food preservatives -- Periodicals; 664
• DOI: 10.1111/jfs.12080
• Languages: English
• ISSNs: 0149-6085

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jfs12080-sec-1001" sec-type="section"> <p>Most illnesses caused by <italic>E</italic><italic>scherichia coli</italic> O157:H7 have been associated with eating undercooked, contaminated meat. In this study, a number of 200 cooked beef doner kebab samples from different restaurants across cities of Tehran and Mashhad were analyzed. After enrichment, selective plating and biochemical tests, the DNA from suspected colonies were extracted. Three multiplex polymerase chain reaction (PCR) assays to confirm the isolates as <italic>E</italic><italic>. coli</italic> O157:H7 and the presence of virulence factors were performed. Specific primers for <italic>cH7</italic> and <italic>rfbO157</italic> genes in the first and for <italic>stx1</italic> and <italic>stx2</italic> genes in the second m‐PCR were used. The third m‐PCR performed using specific primers for <italic>eaeA</italic> and <italic>EhlyA</italic> genes. From 25 nonsorbitol‐fermenting isolates, 21 isolates confirmed as <italic>E</italic><italic>. coli</italic> O157:H7. The results of third m‐PCR showed 12 isolates harboring the <italic>EhlyA</italic> gene, three isolates the <italic>eaeA</italic> gene and three isolates harboring both the <italic>EhlyA</italic> and <italic>eaeA</italic> genes.</p> </sec> <sec id="jfs12080-sec-0001" sec-type="section"> <title>Practical Applications</title> <p>The presence of <italic>E</italic><italic>scherichia coli</italic> O157:H7 in cooked beef doner kebabs could be considered a serious public health hazard. These three multiplex PCR assay are a suitable method for rapid identification of <italic>E</italic><italic>. coli</italic> O157:H7 virulence genes.</p> </sec> </abstract>