Effects of supplementing different ratios of omega‐3 and omega‐6 fatty acids in western‐style diets on cow's milk protein allergy in a mouse model. Issue 11 (19th June 2013)

• Record Type: Journal Article
• Authors: Thang, Cin L.; Boye, Joyce I.; Shi, Hai Ning; Zhao, Xin
• Is Part Of: Molecular nutrition & food research. Volume 57:Issue 11(2013:Nov.)
• Page Start: 2029
• Page End: 2038
• Publication Date: 2013-06-19
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201300061
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr2010-sec-0010" sec-type="section"> <title>Scope</title> <p>This study investigated the effects of supplementing different ratios of omega‐6 and omega‐3 fatty acids (O6H = 10:1, O3O6 = 4:1, and O3H = 1:4) to western‐style diets on cow β‐lactoglobulin (BLG) induced allergic reactions in Balb/c mice.</p> </sec> <sec id="mnfr2010-sec-0020" sec-type="section"> <title>Methods and results</title> <p>Three‐week‐old mice were randomly assigned to three diet groups (<italic>n</italic> = 20/group). At 9 wk of age, half of the mice from each dietary treatment (<italic>n</italic> = 10) were intraperitoneally (i.p.) sensitized with three weekly doses of BLG and alum while the remaining half from each group was sham sensitized (controls). One week after the final sensitization, all mice were orally challenged with BLG. Elevated BLG‐specific serum Igs were observed in all sensitized and challenged mice. IFN‐γ, MCP‐1, and IL‐12p40 concentrations from lymphocytes of mesenteric lymph nodes were highest in O3H mice, compared to O3O6 and O6H mice. O6H mice had the highest IL‐4 concentrations from splenic lymphocytes and a significantly lower rectal temperature after the challenge in comparison to O3O6 and O3H mice.</p> </sec> <sec id="mnfr2010-sec-0030" sec-type="section"> <title>Conclusions</title> <p>Our results suggest that the ω‐3 PUFA rich diets alleviated the severity of allergic reactions, and may modulate immune response toward T helper cell (Th)1‐favoured immune response while the ω‐6 PUFA rich diet exhibited no allergy alleviation with a stronger Th2 polarized immune response.</p> </sec> </abstract>