Phenolic acid concentrations in plasma and urine from men consuming green or black tea and potential chemopreventive properties for colon cancer. Issue 3 (14th January 2013)

• Record Type: Journal Article
• Authors: Henning, Susanne M.; Wang, Piwen; Abgaryan, Narine; Vicinanza, Roberto; de Oliveira, Daniela Moura; Zhang, Yanjun; Lee, Ru‐Po; Carpenter, Catherine L.; Aronson, William J.; Heber, David
• Is Part Of: Molecular nutrition & food research. Volume 57:Issue 3(2013:Mar.)
• Page Start: 483
• Page End: 493
• Publication Date: 2013-01-14
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201200646
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr1903-sec-0010" sec-type="section"> <title>Scope</title> <p>Tea polyphenols are metabolized by the colonic microflora yielding phenolic metabolites, which may contribute to the health benefits of tea. We determined the serum and urine concentrations of phenolic acids, hippuric acid, and polyhydroxyphenyl‐γ‐valerolactones during green tea (GT) and black tea (BT) administration. The effects of (–)‐epigallocatechin gallate (EGCG) and 3, 4‐dihydroxyphenylacetic acid (3, 4‐DHPAA) alone and in combination on bioavailability, intracellular metabolism, and antiproliferative activity were determined in HCT‐116 colon cancer cells.</p> </sec> <sec id="mnfr1903-sec-0020" sec-type="section"> <title>Methods and results</title> <p>The concentration of phenolic metabolites was quantified by HPLC with electrochemical detection and MS. Urine concentrations of 4‐hydroxyphenylacetic acid (4‐HPAA), 3‐hydroxyphenylacetic acid (3‐HPAA), and polyhydroxy‐γ‐valerolactones were increased significantly in men drinking GT compared to control. Urine concentration of 3‐<italic>O</italic>‐methylgallic acid (3OMGA) was significantly increased in men drinking BT compared to control. Serum 3, 4‐DHPAA was significantly increased after consumption of GT and BT and 4‐HPAA after GT consumption. In vitro treatment of HCT‐116 colon cancer cells with 3, 4‐DHPAA and EGCG exhibited an additive antiproliferative effect, while methylation of 3, 4‐DHPAA was significantly decreased. 3OMGA exhibited the strongest antiproliferative activity among the phenolic acids.</p> </sec> <sec id="mnfr1903-sec-0030" sec-type="section"> <title>Conclusion</title> <p>The consumption of both, GT and BT, was associated with a significant increase in urinary and serum phenolic acids.</p> </sec> </abstract>