Plasma B vitamins and LINE‐1 DNA methylation in leukocytes of patients with a history of colorectal adenomas. Issue 4 (7th November 2012)

• Record Type: Journal Article
• Authors: Jung, Audrey Y.; Botma, Akke; Lute, Carolien; Blom, Henk J.; Ueland, Per M.; Kvalheim, Gry; Midttun, Øivind; Nagengast, Fokko; Steegenga, Wilma; Kampman, Ellen; Ulrich, Cornelia M.; Miller, Joshua W.
• Is Part Of: Molecular nutrition & food research. Volume 57:Issue 4(2013:Apr.)
• Page Start: 698
• Page End: 708
• Publication Date: 2012-11-07
• Subjects: Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201200069
• Languages: English
• ISSNs: 1613-4125

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mnfr1864-sec-0010" sec-type="section"> <title>Scope</title> <p>Low concentrations of folate, other B vitamins, and methionine are associated with colorectal cancer risk, possibly by changing DNA methylation patterns. Here, we examine whether plasma concentrations of B vitamins and methionine are associated with methylation of long interspersed nuclear element‐1 (LINE‐1) among those at high risk of colorectal cancer, i.e. patients with at least one histologically confirmed colorectal adenoma (CRA) in their life.</p> </sec> <sec id="mnfr1864-sec-0020" sec-type="section"> <title>Methods and results</title> <p>We used LINE‐1 bisulfite pyrosequencing to measure global DNA methylation levels in leukocytes of 281 CRA patients. Multivariable linear regression was used to assess associations between plasma B vitamin concentrations and LINE‐1 methylation levels. Plasma folate was inversely associated with LINE‐1 methylation in CRA patients, while plasma methionine was positively associated with LINE‐1 methylation.</p> </sec> <sec id="mnfr1864-sec-0030" sec-type="section"> <title>Conclusion</title> <p>This study does not provide evidence that in CRA patients, plasma folate concentrations are positively related to LINE‐1 methylation in leukocytes but does suggest a direct association between plasma methionine and LINE‐1 methylation in leukocytes.</p> </sec> </abstract>