A liquid chromatography/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in Coffea arabica leaves. (26th July 2013)

• Record Type: Journal Article
• Authors: de Sá, Marta; Ferreira, João P; Queiroz, Vagner T; Vilas‐Boas, Luís; Silva, Maria C; Almeida, Maria H; Guerra‐Guimarães, Leonor; Bronze, Maria R
• Is Part Of: Journal of the science of food and agriculture. Volume 94:Number 3(2014:Feb. 15)
• Page Start: 529
• Page End: 536
• Publication Date: 2013-07-26
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6288
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main" id="jsfa6288-abs-0001"> <title>Abstract</title> <sec id="jsfa6288-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jsfa6288-para-0001"> <bold>Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples</bold>.</p> </sec> <sec id="jsfa6288-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="jsfa6288-para-0002"> <bold>In this study a liquid chromatography/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of SA, JA and ABA in <italic>Coffea arabica</italic> (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against <italic>Hemileia vastatrix</italic>. The results showed that the method was specific, linear (<italic>r</italic> ≥ 0.99) in the range 0.125–1.00 µg mL<sup>−1</sup> for JA and ABA and 0.125–5.00 µg mL<sup>−1</sup> for SA, and precise (relative standard deviation ≤11%), and the limit of detection (0.010 µg g<sup>−1</sup> fresh weight) was adequate for quantifying these phytohormones in this type of matrix</bold>.</p> </sec> <sec id="jsfa6288-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="jsfa6288-para-0003"> <bold>In comparison with healthy leaves, those infected with <italic>H. vastatrix</italic> (resistance reaction) displayed an increase in SA level 24 h after inoculation, suggesting the involvement of an SA‐dependent pathway in coffee resistance. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>