Isolation of Bioactive Phytoconstituent from Alpinia galanga L. with Anti-Hyperlipidemic Activity. (1st December 2013)

• Record Type: Journal Article
• Authors: Iyer, Deepa; Sharma, Brajesh K.; Patil, U. K.
• Is Part Of: Journal of dietary supplements. Volume 10:Number 4(2013:Dec.)
• Page Start: 309
• Page End: 317
• Publication Date: 2013-12-01
• Subjects: Dietary supplements -- Periodicals; Dietary Supplements -- Periodicals; 613.205
• DOI: 10.3109/19390211.2013.830674
• Languages: English
• ISSNs: 1939-0211

Abstract

<abstract> <title>ABSTRACT</title> <p>The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract of rhizomes of <italic>Alpinia galanga</italic> L. and its chloroform fraction in Triton-induced hyperlipidemic rats. Bioactivity guided fractionation was followed by chromatographic studies. Flash chromatography was done for the most active fraction resulting in the isolation of 5-(hydroxymethyl) furfural. Animals were administered with i.p. injection of Triton WR 1339 at dose of 400 mg/kg body weight. After 24 hr of Triton administration, the ethanolic extract and its fraction were administered orally at doses of 200 and 400 mg/kg body weight in rats. The treatment was continued for 5 days with a view to see the effect on lipid profile. Serum samples were subjected to biochemical analysis. The study dose dependently inhibited the total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) level, and significantly increased high-density lipoprotein (HDL) level. Phytochemical screening revealed the presence of tannins, coumarins, flavanoids, sterols, and glycosides. Phytochemical investigation of the chloroform fraction of <italic>A. galanga</italic> L. resulted in the isolation of 5-(hydroxymethyl) furfural. UV λmax was found to be 276 nm for the isolated component. Acute treatment caused a stimulatory effect on the HDL level and inhibition in TC and TG elevation induced by triton.</p> </abstract>