A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model. (26th December 2012)

• Record Type: Journal Article
• Authors: Leal, Ricardo Miguel Ferraz; Bourenkov, Gleb; Russi, Silvia; Popov, Alexander N.
• Is Part Of: Journal of synchrotron radiation. Volume 20:Part 1(2013)
• Page Start: 14
• Page End: 22
• Publication Date: 2012-12-26
• Subjects: Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S0909049512049114
• Languages: English
• ISSNs: 0909-0495

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The radiation damage rates to crystals of 15 model macromolecular structures were studied using an automated radiation sensitivity characterization procedure. The diffracted intensity variation with dose is described by a two‐parameter model. This model includes a strong resolution‐independent decay specific to room‐temperature measurements along with a linear increase in overall Debye–Waller factors. An equivalent representation of sensitivity <italic>via</italic> a single parameter, normalized half‐dose, is introduced. This parameter varies by an order of magnitude between the different structures studied. The data show a correlation of crystal radiation sensitivity with crystal solvent content but no dose‐rate dependency was detected in the range 0.05–300 kGy s⁻¹. The results of the crystal characterization are suitable for either optimal planning of room‐temperature data collection or <italic>in situ</italic> crystallization plate screening experiments.</p> </abstract>