A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model. (26th December 2012) - Leal, Ricardo Miguel Ferraz
Abstract The radiation damage rates to crystals of 15 model macromolecular structures were studied using an automated radiation sensitivity characterization procedure. The diffracted intensity variation with dose is described by a two‐parameter model. This model includes a strong resolution‐independent decay specific to room‐temperature measurements along with a linear increase in overall Debye–Waller factors. An equivalent representation of sensitivity via a single parameter, normalized half‐dose, is introduced. This parameter varies by an order of magnitude between the different structures studied. The data show a correlation of crystal radiation sensitivity with crystal solvent content but no dose‐rate dependency was detected in the range 0.05–300 kGy s−1. The results of the crystal characterization are suitable for either optimal planning of room‐temperature data collection or in situ crystallization plate screening experiments.