Bridelia ferruginea Promotes Reactive Oxygen Species Detoxification in N-Nitrosodiethylamine-Treated Rats. (1st September 2013)

• Record Type: Journal Article
• Authors: Ajiboye, T. O.; Abdussalam, F. A.; Adeleye, A. O.; Iliasu, G. A.; Ariyo, F. A.; Adediran, Z. A.; Raji, K. O.; Raji, H. O.
• Is Part Of: Journal of dietary supplements. Volume 10:Number 3(2013:Sep.)
• Page Start: 210
• Page End: 228
• Publication Date: 2013-09-01
• Subjects: Dietary supplements -- Periodicals; Dietary Supplements -- Periodicals; 613.205
• DOI: 10.3109/19390211.2013.822451
• Languages: English
• ISSNs: 1939-0211

Abstract

<abstract> <title>ABSTRACT</title> <p>This study was aimed at investigating the <italic>in vitro</italic> and <italic>in vivo</italic> reactive oxygen species (ROS) scavenging and detoxification potentials of aqueous extract of <italic>Bridelia ferruginea</italic> (<italic>B. ferruginea</italic>). Reactive oxygen species scavenging potentials of the aqueous leaf extract of <italic>B. ferruginea</italic> (0.2–1.0 mg/ml) was investigated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide ion, hydrogen peroxide, hydroxyl radical, and ferric ion reducing system. The detoxification of ROS was evaluated in N-nitrosodiethylamine-induced redox imbalance in the liver of rats. <italic>B. ferruginea</italic> extract at 1.0 mg/ml scavenged the DPPH, superoxide ion, hydrogen peroxide, and hydroxyl radical at 87%, 83%, 92%, and 86%, respectively, it also reduced ferric ion significantly. ROS detoxifying enzymes (superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, and glucose 6-phosphate dehydrogenase) activities were significantly (<italic>p</italic> < .05) induced by <italic>B. ferruginea.</italic> These inductions significantly ( <italic>p</italic> < .05) attenuated the N-nitrosodiethylamine-mediated decrease in ROS detoxifying enzymes and compared favorably with Vitamin C. N-nitrosodiethylamine-mediated elevation in the concentrations of malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl, and percentage DNA fragmentation were significantly (<italic>p</italic> < .05) lowered by <italic>B. ferruginea</italic> extract. Overall, the results of this study show that <italic>B. ferruginea</italic> leaf extract possess ROS scavenging and detoxification potentials.</p> </abstract>