A comparison of gene expression of Listeria monocytogenes in vitro and in the soft cheese Crescenza. (29th October 2012)

• Record Type: Journal Article
• Authors: Alessandria, Valentina; Rantsiou, Kalliopi; Dolci, Paola; Zeppa, Giuseppe; Cocolin, Luca
• Is Part Of: International journal of dairy technology. Volume 66:Number 1(2013:Feb.)
• Page Start: 83
• Page End: 89
• Publication Date: 2012-10-29
• Subjects: Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12008
• Languages: English
• ISSNs: 1364-727X

Abstract

<abstract abstract-type="main" id="idt12008-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This study focuses on the expression analysis of virulence and stress response genes (<italic>plcA</italic>, <italic> hly</italic>, <italic> iap</italic> and <italic>sigB</italic>) in 11 different strains of <italic>Listeria monocytogenes</italic>. The first step of this study considered an <italic>in vitro</italic> analysis in synthetic laboratory medium. According to statistical analysis, significant differences emerged for genes <italic>sigB</italic> and <italic>plcA</italic>. Subsequently, a soft cheese at two temperatures (4 and 12 °C) and for different times (24 and 48 h) was evaluated. Significant expression differences emerged for the condition at 12 °C after 48 h. No association could be observed between gene expression profile and origin of the different strains.</p> </abstract>