An enzyme‐linked immunosorbent assay for the measurement of plasma flavonoids in mice fed apigenin‐C‐glycoside. (29th April 2013)

• Record Type: Journal Article
• Authors: Ninfali, Paolino; Dominici, Sabrina; Angelino, Donato; Gennari, Lorenzo; Buondelmonte, Costantina; Giorgi, Luca
• Is Part Of: Journal of the science of food and agriculture. Volume 93:Number 12(2013:Sep. 15)
• Page Start: 3087
• Page End: 3093
• Publication Date: 2013-04-29
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.6143
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jsfa6143-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p> <bold>In the <italic>Chenopodiaceae</italic> family, the apigenin flavonoids vitexin‐2‐<italic>O</italic>‐xyloside (VOX) and vitexin‐2‐<italic>O</italic>‐rhamnoside (VOR) are important chemopreventive components. To investigate their bioavailability in <italic>in vivo</italic> animal studies an enzyme‐linked immunosorbent assay (ELISA) method has been developed</bold>.</p> </sec> <sec id="jsfa6143-sec-0002" sec-type="section"> <title>RESULTS</title> <p> <bold>The ELISA was based on polyclonal antibodies elicited in mice by injecting, as an immunogen, 4′, 6″‐<italic>O</italic>‐biapigenin (hinokiflavone, HF) conjugated to bovine serum albumin (BSA–HF). A second immunogen was synthesised by coupling an equimolar mixture of VOX and VOR to BSA (BSA–F1). The BSA–HF elicited a significant antibody response, due to 17 HF hapten groups, coupled to each BSA molecule, whereas BSA–F1 provided a very low antigenicity in respect to control animals. Antiserum raised against BSA–HF showed an antibody titre of 1:1600. Antibodies were found to be specific for the flavonols. Our results show that VOX and its metabolic products reached the concentration of 3.42 ± 0.72 µg mL<sup>−1</sup> in plasma of VOX fed animals, at the net of the control value</bold>.</p> </sec> <sec id="jsfa6143-sec-0003" sec-type="section"> <title>CONCLUSIONS</title> <p> <bold>By using the ELISA, the concentration of apigenin flavonoids and their metabolites can be detected in VOX‐ or VOR‐supplemented animals. The assay represents a useful tool for rapid screening to compare bioavailability of apigenin flavonoids in respect to control animals. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>