Genetic diversity and antimicrobial resistance of Yersinia enterocolitica isolated from pigs and humans in Lithuania. (12th December 2012)

• Record Type: Journal Article
• Authors: Novoslavskij, Aleksandr; Kudirkienė, Eglė; Marcinkutė, Audronė; Bajoriūnienė, Almina; Korkeala, Hannu; Malakauskas, Mindaugas
• Is Part Of: Journal of the science of food and agriculture. Volume 93:Number 8(2013:Jun. 15)
• Page Start: 1858
• Page End: 1862
• Publication Date: 2012-12-12
• Subjects: Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.5980
• Languages: English
• ISSNs: 0022-5142

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jsfa5980-sec-0001" sec-type="section"> <title>Background</title> <p> <bold>Yersiniosis is one of the three leading foodborne zoonoses in Lithuania, and the incidence of 12.86 per 100 000 population was the highest among EU member states in 2010. Contaminated pig carcasses and subsequently undercooked pig meat are considered to be the primary transmission vehicle of enteropathogenic <italic>Y. enterocolitica</italic> to consumers. With the aim of evaluating pigs as a possible source of human yersiniosis in Lithuania, this study investigated the genetic diversity of <italic>Y. enterocolitica</italic> isolated from pigs and human cases of yersiniosis. In addition, the antimicrobial resistance of selected isolates from both sources was compared</bold>.</p> </sec> <sec id="jsfa5980-sec-0002" sec-type="section"> <title>Results</title> <p> <bold>In total, 83 <italic>Y. enterocolitica</italic> strains were characterised using pulsed field gel electrophoresis. Overall, 68% of <italic>Y. enterocolitica</italic> 4/O:3 pulsotypes found in human clinical samples were identical to 81% of pulsotypes found in the pig production chain. <italic>Yersinia enterocolitica</italic> pulsotype II was confirmed as the dominant pulsotype in the pig production chain and was identical to nine of 19 <italic>Y. enterocolitica</italic> strains found in humans. All tested <italic>Y. enterocolitica</italic> 4/O:3 strains were resistant to ampicillin and erythromycin and sensitive to ciprofloxacin. Of the strains studied, 5% were resistant to tetracycline and streptomycin</bold>.</p> </sec> <sec id="jsfa5980-sec-0003" sec-type="section"> <title>Conclusion</title> <p> <bold>This study showed that pigs may be the main source of human yersiniosis in Lithuania. In addition, <italic>Y. enterocolitica</italic> 4/O:3 strains isolated from the pig production chain and from yersiniosis patients shared similar resistance to different antimicrobials.© 2012 Society of Chemical Industry</bold> </p> </sec> </abstract>