Food allergens : methods and protocols /: methods and protocols. (2017)

• Record Type: Book
• Further Information: Note: Edited by Jing Lin, Bioinformatics Institute, A*STAR, Singapore; Institute of High Performance Computing, A*STAR, Singapore; Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA, Marcos Alcocer, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK.
• Editors: MD, Lin, Jing; Alcocer, Marcos J. C
• Contents: Preface… Table of Contents… Contributing Authors… 1: Overview of the Commonly used Methods for Food Allergens Jing Lin and Marcos J.C. Alcocer Part I Food Allergen Purification and Production 2: Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques Barbara Cases, Carlos Pastor-Vargas, and Marina Perez-Gordo 3: Recombinant Allergen Production in E. Coli Changqi Liu, LeAnna N. Willison, and Shridhar K. Sathe 4: Recombinant Allergens Production in Yeast Maria Neophytou and Marcos J.C. Alcocer Part II Food Allergen Discovery, Detection, and Quantification 5: 2D-Electrophoresis and Immunoblotting in Food Allergy Galina Grishina, Luda Bardina, and Alexander Grishin 6: Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS) Fernando de la Cyesta, Gloria Alvarez-Llamas, and Maria G. Barderas 7: Enzyme-Linked Immunosorbent Assay (ELISA) George N. Konstantinou 8: Detection of Food Allergens by Taqman Real-Time PCR Methodology Aina García, Raquel Madrid, Teresa García, Rosario Martín, and Isabel González 9: Detection of Food Allergens by Phage Displayed Produced Antibodies Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, and Teresa García 10: Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis Nuzul N. Jambari, XiaoWei Wang, and Marcos J.C. Alcocer 11: Basophil Degranulation Assay Madhan Masilamani, Mohanapriya Kamalakannan, and Hugh A. Sampson 12: Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy Eman Ali Ali, Ryosuke Nakamura, and Franco H. Falcone Part III Allergenic Epitope Mapping 13: Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid Sara Benedé, Rosina López-Fandiño, and Elena Molina 14: IgE Epitope Mapping using Peptide Microarray Immunoassay Jing Lin and Hugh A. Sampson 15: T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens Madhan Masilamani, Mariona Pascal, and Hugh A. Sampson 16: Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens Luis Diego Archila and William W. Kwok 17: T-Cell Epitope Prediction George N. Konstantinou 18: An Overview of Bioinformatics Tools and Resources in Allergy Zhiyan Fu and Jing Lin Part IV Methods Currently being Developed and Future Development 19: The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells Ashfaq Ghumra and Marcos J.C. Alcocer 20: Recent Advances in the Detection of Allergens in Foods Silvia de la Crus, Inés López-Calleja, Rosario Martín, Isabel González, Marcos J.C. Alcocer, and Teresa García.
• Publisher Details: New York : Humana Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (299 pages)
• Subjects: 616.97/5; Medicine; Food allergy; Food allergy -- Laboratory manuals; Food Hypersensitivity -- immunology; Allergens; Food; Food allergy; Immunology; Medical -- Immunology; Immunology; Laboratory Manuals; Laboratory manuals
• Languages: English
• ISBNs: 9781493969258
• Related ISBNs: 9781493969234
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.