Guide to protein purification. (2009)

• Record Type: Book
• Further Information: Note: Edited by Richard R. Burgess, Murray P. Deutscher.
• Other Names: Burgess, Richard R; Deutscher, Murray P
• Contents: Why purify enzymes? / A. Kornberg -- Strategies and considerations for protein purifications / S. Linn -- Use of bioinformatics in planning a protein purification / R.R. Burgess -- Preparing a purification summary table / R.R. Burgess -- Setting up a laboratory / M.P. Deutscher -- Buffers: principles and practice / V.S. Stoll and J.S. Blanchard -- Measurement of enzyme activity / T.K. Harris and M.M. Keshwani -- Quantitation of protein / J.E. Noble and M.J. Bailey -- Concentration of proteins and removal of solutes / D.R. Evans, J.K. Romero and M. Westoby -- Maintaining protein stability / M.P. Deutscher -- Selecting an appropriate method for expressing a recombinant protein / W.H. Brondyk -- Bacterial systems for production of heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart -- Expression in the yeast Pichia pastoris / J.M. Cregg [and others] -- Baculovirus-insect cell expression systems / D.L. Jarvis -- Recombinant protein production by transient gene transfer into Mammalian cells / S. Geisse and C. Fux -- Tagging for protein expression / A. Malhotra -- Refolding solubilized inclusion body proteins / R.R. Burgess -- Advances in preparation of biological extracts for protein purification / A.C. Grabski -- Isolation of subcellular organelles and structures / U. Michelsen and J. von Hagen -- Protein precipitation techniques / R.R. Burgess -- Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins / M.P. Deutscher -- Ion-exchange chromatography / A. Jungbauer and R. Hahn -- Gel filtration / E. Stellwagen -- Protein chromatography on hydroxyapatite columns / L.J. Cummings, M.A. Snyder and K. Brisack -- Theory and use of hydrophobic interaction chromatography in protein purification applications / J.T. McCue -- Affinity chromatography: general methods / M. Urh, D. Simpson and K. Zhao -- Immobilized-metal affinity chromatography (IMAC): a review / H. Block [and others] -- Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography / N.E. Thompson [and others] -- One-dimensional gel electrophoresis / D.E. Garfin -- Isoelectric focusing and two-dimensional gel electrophoresis / D.B. Friedman, S. Hoving and R. Westermeier -- Protein gel staining methods: an introduction and overview / T.H. Steinberg -- Elution of proteins from gels / R.R. Burgess -- Performing and optimizing Western blots with an emphasis on chemiluminescent detection / A. Alegria-Schaffer, A. Lodge and K. Vattem -- Detergents: an overview / D. Linke -- Purification of membrane proteins / S.H. Lin and G. Guidotti -- Purification of recombinant G-protein-coupled receptors / R. Grisshammer -- Cell-free translation of integral membrane proteins into unilamelar liposomes / M.A. Goren [and others] -- Determination of protein purity / D.G. Rhodes and T.M. Laue -- Determination of size, molecular weight, and presence of subunits / D.G. Rhodes, R.E. Bossio and T.M. Laue -- Identification and quantification of protein posttranslational modifications / A.R. Farley and A.J. Link -- Parallel methods for expression and purification / S.A. Lesley -- Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example / A. Yan and P.J. Kiley -- Rethinking your purification procedure / M.P. Deutscher -- Important but little known (or forgotten) artifacts in protein biochemistry / R.R. Burgess.
• Edition: 2nd ed
• Publisher Details: Amsterdam Boston : Elsevier/Academic Press
• Publication Date: 2009
• Extent: 1 online resource (liii, 851 pages, [8] pages of plates), illustrations (some color)
• Subjects: 547.7; Proteins -- Purification; Proteins -- isolation & purification; Natural history; Science; SCIENCE -- Chemistry -- Organic; Proteins -- Purification; Proteine; Reinigung; Electronic books
• Languages: English
• ISBNs: 9780080923178; 0080923178
• Related ISBNs: 9780123745361; 0123745365; 9780123749789; 0123749786
• Notes: Note: Includes bibliographical references and indexes.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.