An in vitro assessment for evaluating the efficiency of β‐d‐mannuronic acid (M2000) in myelodysplastic syndrome. Issue 8 (10th December 2018)

• Record Type: Journal Article
• Authors: Bakhtiari, Tahereh; Ghaderi, Afshin; Safaee Nodehi, Sayyed Reza; Aghazadeh, Zahra; Tofighi Zavareh, Farzaneh; Jafarnezhad‐Ansariha, Fahimeh; Barati, Anis; Mirshafiey, Abbas
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 8(2019:Aug.)
• Page Start: 12971
• Page End: 12977
• Publication Date: 2018-12-10
• Subjects: β‐D‐mannuronic acid (M2000) -- immunosuppression -- immunomodulation; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27966
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: β‐d ‐Mannuronic acid (M2000), a novel non‐steroidal anti‐inflammatory drug (NSAID) with immunosuppressive properties, has been previously shown to exhibit potential therapeutic effects on autoimmune diseases. Immunosuppression therapy has been a standard approach for myelodysplastic syndrome (MDS) for many years. We evaluated the effect of M2000 on isolated peripheral blood mononuclear cells (PBMCs) from patients with MDS. The PBMCs were isolated from 13 patients with MDS and 13 normal donors. The cells were then treated with low, moderate, and high doses of M2000 and diclofenac as a control group. The level of interleukin (IL)‐6, tumor necrosis factor alpha (TNF‐α), IL‐3, granulocyte colony‐stimulating factor (G‐CSF) gene expression and the serum level of IL‐6 and TNF‐α production were evaluated by real‐time polymerase chain reaction and enzyme‐linked immunosorbent assay methods, respectively. Our findings indicated a significant reduction in the production of IL‐6 and TNF‐α as inflammatory cytokines. Furthermore, the level of G‐CSF gene expression was significantly increased. In conclusion, M2000, a newly designed NSAID, has a remarkable effect on isolated PBMC in patients with MDS, which might bring a potential hope for its oral administrations in these patients. Abstract : M2000, a newly designed NSAID, has a remarkable effect on isolated peripheral blood mononuclear cell in myelodysplastic patients. It could increase the level of interleukin (IL)‐3, G‐CSF and reduce the production of IL‐6 and tumor necrosis factor alpha.