A Rare Mutation in SPLUNC1 Affects Bacterial Adherence and Invasion in Meningococcal Disease. (1st July 2019)

• Record Type: Journal Article
• Authors: Mashbat, Bayarchimeg; Bellos, Evangelos; Hodeib, Stephanie; Bidmos, Fadil; Thwaites, Ryan S; Lu, Yaxuan; Wright, Victoria J; Herberg, Jethro A; Klobassa, Daniela S; Walton, William G; Zenz, Werner; Hansel, Trevor T; Nadel, Simon; Langford, Paul R; Schlapbach, Luregn J; Li, Ming-Shi; Redinbo, Matthew R; Di, Y Peter; Levin, Michael; Sancho-Shimizu, Vanessa
• Is Part Of: Clinical infectious diseases. Volume 70:Number 10(2020)
• Page Start: 2045
• Page End: 2053
• Publication Date: 2019-07-01
• Subjects: severe infectious disease -- meningococcal disease -- mucosal immunity -- human genetics -- sepsis; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciz600
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: Background: Neisseria meningitidis (Nm) is a nasopharyngeal commensal carried by healthy individuals. However, invasive infections occurs in a minority of individuals, with devastating consequences. There is evidence that common polymorphisms are associated with invasive meningococcal disease (IMD), but the contributions of rare variants other than those in the complement system have not been determined. Methods: We identified familial cases of IMD in the UK meningococcal disease study and the European Union Life-Threatening Infectious Disease Study. Candidate genetic variants were identified by whole-exome sequencing of 2 patients with familial IMD. Candidate variants were further validated by in vitro assays. Results: Exomes of 2 siblings with IMD identified a novel heterozygous missense mutation in BPIFA1 / SPLUNC1 . Sequencing of 186 other nonfamilial cases identified another unrelated IMD patient with the same mutation. SPLUNC1 is an innate immune defense protein expressed in the nasopharyngeal epithelia; however, its role in invasive infections is unknown. In vitro assays demonstrated that recombinant SPLUNC1 protein inhibits biofilm formation by Nm, and impedes Nm adhesion and invasion of human airway cells. The dominant negative mutant recombinant SPLUNC1 (p.G22E) showed reduced antibiofilm activity, increased meningococcal adhesion, and increased invasion of cells, compared with wild-type SPLUNC1. Conclusions: A mutation in SPLUNC1 affecting mucosal attachment, biofilm formation, and invasion of mucosal epithelial cells is a new genetic cause of meningococcal disease. Abstract : Severe meningococcal disease may result from single-gene inborn errors of immunity, affecting bacterial colonization, biofilm formation, and invasion. Our findings demonstrate the host protective role SPLUNC1 plays in maintaining mucosal immunity in the upper respiratory tract, and preventing invasive disease.