The SARS-CoV-2 UTR's Intrudes Host RBP's and Modulates Cellular Splicing. (5th April 2023)

• Record Type: Journal Article
• Authors: Singh, Anjali; Pandey, Kush Kumar; Agrawal, Shubham Kumar; Srivastava, Rupesh K.; Bhattacharyya, Sankar; Verma, Bhupendra
• Other Names: Verma Subhash C. Academic Editor.
• Is Part Of: Advances in virology. Volume 2023(2023)
• Publication Date: 2023-04-05
• Subjects: Virology -- Periodicals; 616.9101
• DOI: 10.1155/2023/2995443
• Languages: English
• ISSNs: 1687-8639

Abstract

Abstract : SARS-CoV-2 is a novel coronavirus that causes a potentially fatal respiratory disease known as coronavirus disease (COVID-19) and is responsible for the ongoing pandemic with increasing mortality. Understanding the host-virus interaction involved in SARS-CoV-2 pathophysiology will enhance our understanding of the mechanistic basis of COVID-19 infection. The characterization of post-transcriptional gene regulatory networks, particularly pre-mRNA splicing, and the identification and characterization of host proteins interacting with the 5′ and 3′UTRs of SARS-CoV-2 will improve our understanding of post-transcriptional gene regulation during SARS-CoV-2 pathogenesis. Here, we demonstrate that either SARS-CoV-2 infection or exogenous overexpression of the 5′ and 3'UTRs of the viral genomic RNAs, results in reduced mRNA levels possibly due to modulation of host cell pre-mRNA splicing. Further, we have investigated the potential RNA-binding proteins interacting with the 5′ and 3′UTRs, using in-silico approaches. Our results suggest that 5′ and 3′UTRs indeed interact with many RNA-binding proteins. Our results provide a primer for further investigations into the UTR-mediated regulation of splicing and related molecular mechanisms in host cells.