Cyclic nucleotide‐dependent ionic currents in olfactory receptor neurons of the hawkmoth Manduca sexta suggest pull–push sensitivity modulation. (8th July 2021)

• Record Type: Journal Article
• Authors: Dolzer, Jan; Schröder, Katrin; Stengl, Monika
• Is Part Of: European journal of neuroscience. Volume 54:Number 3(2021)
• Page Start: 4804
• Page End: 4826
• Publication Date: 2021-07-08
• Subjects: cAMP -- cGMP -- insects -- metaplasticity -- olfactory transduction; Nervous system -- Periodicals; 612.8
• DOI: 10.1111/ejn.15346
• Languages: English
• ISSNs: 0953-816X

Abstract

Abstract: Olfactory receptor neurons (ORNs) of the hawkmoth Manduca sexta sensitize via cAMP‐ and adapt via cGMP‐dependent mechanisms. Perforated patch clamp recordings distinguished 11 currents in these ORNs. Derivatives of cAMP and/or cGMP antagonistically affected three of five K + currents and two non‐specific cation currents. The Ca 2+ ‐dependent K + current IK(Ca 2+ ) and the sensitive pheromone‐dependent K + current IK(cGMP−), which both express fast kinetics, were inhibited by 8bcGMP, while a slow K + current, IK(cGMP+), was activated by 8bcGMP. Furthermore, application of 8bcAMP blocked slowly activating, zero mV‐reversing, non‐specific cation currents, ILL and Icat(PKC?), which remained activated in the presence of 8bcGMP. Their activations pull the membrane potential towards their 0‐mV reversal potentials, in addition to increasing intracellular Ca 2+ levels voltage‐ and ILL ‐dependently. Twenty minutes after application, 8bcGMP blocked a TEA‐independent K + current, IK(noTEA), and a fast cation current, Icat(nRP), which both shift the membrane potential to negative values. We conclude that conditions of sensitization are maintained at high levels of cAMP, via specific opening/closure of ion channels that allow for fast kinetics, hyperpolarized membrane potentials, and low intracellular Ca 2+ levels. In contrast, adaptation is supported via cGMP, which antagonizes cAMP, opening Ca 2+ ‐permeable channels with slow kinetics that stabilize depolarized resting potentials. The antagonistic modulation of peripheral sensory neurons by cAMP or cGMP is reminiscent of pull–push mechanisms of neuromodulation at central synapses underlying metaplasticity. Abstract : Olfactory receptor neurons express circadian rhythms in intracellular cAMP levels peaking during the moth's activity phase at night (black bar; dark shade) while cAMP and cGMP are antagonistically controlled via circadian changes in Ca 2+ levels. Thus, for intermittent odour responses, different sets of second messenger‐dependent ion channels are available, with fastest kinetics at night when high cAMP, low cGMP‐, and low Ca 2+ levels predominate, and slowest kinetics at maximal cGMP and Ca 2+ levels during sleep.