Histological, transmission electron microscopic, and immunohistochemical study of the adrenal gland in the Persian squirrel (Sciurus anomalus). Issue 2 (23rd September 2021)

• Record Type: Journal Article
• Authors: Johnny, Mahshid; Goodarzi, Nader; Bazm, Mohsen Akbari
• Is Part Of: Microscopy research and technique. Volume 85:Issue 2(2022)
• Page Start: 738
• Page End: 747
• Publication Date: 2021-09-23
• Subjects: adrenal gland -- histochemistry -- immunohistochemistry -- Persian squirrel -- transmission electron microscope; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23945
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: This research was aimed to present the histological and ultrastructure properties of the adrenal gland in the Persian squirrel. Two male and female animals were included in the study. The adrenal gland was bean‐shaped and located on the cranial pole of kidney. The enveloping capsule was dense connective tissue that reacted positively with Periodic‐Acid Schiff (PAS) and Masson trichrome stainings. The parenchyma of the gland consisted of two‐part, namely cortex and medulla; the cortex had three layers: zona glomerulosa (ZG), zona fasciculata (ZF), and zona reticularis (ZR). The cells of the ZG were mainly spherical and ovoid with circular arrangement and few lipid droplets in TEM micrographs. The cells of the ZF were columnar and spherical that were arranged in cord‐like rows. Transmission electron microscopy (TEM) indicated conspicuous lipid droplets and mitochondria in this zone. The cells of the ZR were arranged in a tangled networks and were almost similar to those in the ZF. TEM images showed fewer lipid vesicles in the ZR compared to the ZF and ZG. Chromaffin cells were located in the medulla of the adrenal gland in two layers. TEM images showed that some of them were smaller and contained fewer secretory granules; other cells were larger and contained more electron‐dense secretory granules. Immunofluorescence staining showed that steroidogenic factor 1 (SF1) expressed from cortex to the corticomedullary junction (CMJ) and tyrosine hydroxylase (TH) expressed in the medulla. In conclusion, the results indicated both similarities and differences between the adrenal gland of the Persian squirrel and other animals such as mammals and rodents. Abstract : The adrenal gland was consisted of cortex and medulla; the cortex was composed of zona glomerulosa (ZG), zona fasciculata (ZF), and zona reticularis (ZR) The cells of the ZG were mainly spherical and ovoid with few lipid droplets in TEM micrographs. The cells of the ZF and ZR were columnar and spherical with conspicuous lipid droplets. Two types of chromaffin cells, namely epinephrine and norepinephrine cells, with fewer and more secretory granules were seen in TEM micrographs Immunofluorescence staining showed steroidogenic factor 1 (SF1) which was expressed from cortex to the corticomedullary junction (CMJ) and tyrosine hydroxylase (TH) was detected in the medullary cells.