Characterization of Campylobacter jejuni–Neutrophil Interactions. Issue 11 (22nd November 2021)

• Record Type: Journal Article
• Authors: Callahan, Sean M.; Hancock, Trevor J.; Johnson, Jeremiah G.
• Is Part Of: Current protocols. Volume 1:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-22
• Subjects: Campylobacter -- host‐microbe interactions -- infection -- neutrophil -- pathogenesis; Life sciences -- Laboratory manuals -- Periodicals; Biology -- Laboratory manuals -- Periodicals; Life sciences -- Technique -- Periodicals; Biology -- Technique -- Periodicals; 570.028
• DOI: 10.1002/cpz1.294
• Languages: English
• ISSNs: 2691-1299

Abstract

Abstract: Campylobacter jejuni is the leading cause of bacterial‐derived gastroenteritis worldwide, infecting 96 million individuals annually. During infection, inflammation and tissue pathology occur in the lower gastrointestinal tract, including the recruitment of leukocytes. Neutrophils are the most abundant leukocyte in humans, and recruitment is associated with bacterial infections and the development of various inflammatory disorders, including inflammatory bowel disease. Neutrophils possess three main antibacterial functions: phagocytosis and degradation of microbes, degranulation to release antimicrobial proteins, and extrusion of neutrophil extracellular traps (NETs). Because neutrophils are recruited to the site of C. jejuni infection and they are associated with damaging inflammation in other diseases, it is imperative to understand the immunopathology that occurs during C. jejuni infection and thoroughly study the neutrophil response to the pathogen. Detailed protocols for human and ferret neutrophil isolations, neutrophil gentamicin protection assay, neutrophil activation flow cytometry assay, NET induction and quantification, and neutrophil western blot analysis are included in this article. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Isolation of human and ferret neutrophils Basic Protocol 2 : Neutrophil gentamicin protection assay Basic Protocol 3 : Neutrophil activation flow cytometry analyses Basic Protocol 4 : Neutrophil extracellular trap induction and quantification Basic Protocol 5 : Western blot detection of neutrophil‐derived antimicrobial proteins