Ultrasensitive Detection of Dopamine, IL‐6 and SARS‐CoV‐2 Proteins on Crumpled Graphene FET Biosensor. Issue 11 (28th August 2021)

• Record Type: Journal Article
• Authors: Hwang, Michael Taeyoung; Park, Insu; Heiranian, Mohammad; Taqieddin, Amir; You, Seungyong; Faramarzi, Vahid; Pak, Angela A.; van der Zande, Arend M.; Aluru, Narayana R.; Bashir, Rashid
• Is Part Of: Advanced materials technologies. Volume 6:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-08-28
• Subjects: crumpled graphene FET biosensor -- COVID‐19 antigen test -- COVID‐19 proteins detection -- dopamine detection -- S‐ and N‐protein; Materials science -- Periodicals; Technological innovations -- Periodicals; Materials science; Technological innovations; Periodicals; 620.1105
• DOI: 10.1002/admt.202100712
• Languages: English
• ISSNs: 2365-709X

Abstract

Abstract: Universal platforms for biomolecular analysis using label‐free sensing modalities can address important diagnostic challenges. Electrical field effect‐sensors are an important class of devices that can enable point‐of‐care sensing by probing the charge in the biological entities. Use of crumpled graphene for this application is especially promising. It is previously reported that the limit of detection (LoD) on electrical field effect‐based sensors using DNA molecules on the crumpled graphene FET (field‐effect transistor) platform. Here, the crumpled graphene FET‐based biosensing of important biomarkers including small molecules and proteins is reported. The performance of devices is systematically evaluated and optimized by studying the effect of the crumpling ratio on electrical double layer (EDL) formation and bandgap opening on the graphene. It is also shown that a small and electroneutral molecule dopamine can be captured by an aptamer and its conformation change induced electrical signal changes. Three kinds of proteins were captured with specific antibodies including interleukin‐6 (IL‐6) and two viral proteins. All tested biomarkers are detectable with the highest sensitivity reported on the electrical platform. Significantly, two COVID‐19 related proteins, nucleocapsid (N‐) and spike (S‐) proteins antigens are successfully detected with extremely low LoDs. This electrical antigen tests can contribute to the challenge of rapid, point‐of‐care diagnostics. Abstract : The crumpled graphene field‐effect transistor‐based biosensing of important biomarkers are reported. Dopamine and three kinds of proteins, which are interleukin‐6 and two COVID‐19 related proteins, are captured with specific aptamer and antibodies. All tested biomarkers are detectable with the highest sensitivity reported. The crumpling effects of the electrical double layer formation and bandgap opening on the graphene are studied.