Drug Resistance in Tuberculous Lymphadenitis: Molecular Characterization. (29th March 2023)

• Record Type: Journal Article
• Authors: Assefa, Gebeyehu; Desta, Kassu; Araya, Shambel; Girma, Selfu; Hailu, Elena; Mihret, Adane; Hailu, Tsegaye; Tilahun, Melaku; Diriba, Getu; Dagne, Biniyam; Atnafu, Abay; Endalafer, Nigatu; Abera, Adugna; Bekele, Shiferaw; Mengistu, Yordanos; Bobosha, Kidist; Aseffa, Abraham
• Other Names: Ivanyi Juraj Academic Editor.
• Is Part Of: Tuberculosis research and treatment. Volume 2023(2023)
• Publication Date: 2023-03-29
• Subjects: Tuberculosis -- Periodicals; Tuberculosis -- Prevention -- Periodicals; Tuberculosis -- Treatment -- Periodicals; Tuberculosis -- Social aspects -- Periodicals; 616.995005
• DOI: 10.1155/2023/3291538
• Languages: English
• ISSNs: 2090-150X

Abstract

Abstract : Background . Drug-resistant tuberculosis (TB) epidemic in high-TB-incidence countries, particularly Ethiopia, remains a significant challenge. As a result, we investigated the drug resistance, common gene mutation, and molecular characterization of mycobacterial isolates from patients with suspected tuberculous lymphadenitis (TBLN). Methodology . A cross-sectional study of 218 FNA samples from TBLN patients inoculated on Lowenstein-Jensen media was carried out. The culture isolates were identified as MTB by polymerase chain reaction (PCR) and the difference-9 (RD9) test region. In addition, the GenoType MTBDR plus assay tested the first and second-line MTB drugs, and the spoligotyping strain-dependent polymorphism test was determined. Results . Among the 50 culture-positive isolates, 14% (7/50) had drug resistance caused by a gene mutation. Out of these, 4 (8%) isolates were mono-resistant to isoniazid drug, which is caused by a gene mutation in katG in the region of interrogated at codon 315 in the amino acid sequence of S315T1, and 3 (6%) isolates were resistant to both rifampicin and isoniazid drugs. The mutation was observed for katG (at codon 315 with a change in the sequence of amino acid S315T) and rpoB (at codon 530–533 with a change in the sequence of amino acid S531L (S450L)) genes. The most prevalent spoligotypes were orphan and SIT53 strains. Conclusion . The predominance of INH mono-resistance poses a critical risk for the potential development of MDR-TB, as INH mono-resistance is a typical pathway to the occurrence of MDR-TB. The orphan and SIT53 (T) strains were the most common in the study area, and a drug-resistant strain caused by a common gene mutation could indicate the transmission of clonal-resistant strains in the community.